What is 'aLeaves'?
aLeaves
allows you to collect homologs of
the sequence of your interest. Those sequences can be passed on to the
web server for sophisticated multiple alignment, and then to tree
building. This initial process one can perform here is just like
collecting
leaves
(termini of trees) from diverse
animals.
This is why we designated this tool '
aLeaves'.
Start the search
(powered by NCBI Blast)
Enter
your "query" peptide
sequence in the fasta
format (example):
Or upload
a file:
Select
database (one or more) (number
of sequences in parentheses):
1. Human -
Refseq
(62,342)
[detail]
2. Human - Ensembl
(116,646)
[detail]
3*. Non-human eutherians - Ensembl
(2,260,241)
[detail]
4. Non-eutherian mammals - Ensembl
(122,040)
[detail]
5. Non-mammalian
vertebrates
- Ensembl
(2,766,415)
[detail]
6. Cartilaginous fishes and cyclostomes
(364,571)
[detail]
7*
. All vertebrate
entries except mammalians in NCBI Protein (7,537,596)
[detail]
8. Invertebrate deuterostomes
(250,212)
[detail]
9. Arthropods
(2,106,748)
[detail]
10. Nematodes
(779,022)
[detail]
11. Other protostomes
(644,150)
[detail]
12. Non-bilaterian
metazoans (cnidarians, placozoan
& poriferan)
(644,150)
[detail]
13*
. All
metazoan
entries except vertebrates in NCBI Protein (11,024,593)
[detail]
(Caution: selecting the
database marked with * will largely slow down the search)
Number of homologs to collect:
(This
number should not exceed 10000)
Threshold E-value:
Set low complexity
filter or not [
help
of NCBI Blast]
:
Note:
Please confirm that the output of this search will later be transferred
to the MAFFT server at Osaka Univ. for downstream processing.
Currently, searches are only executed in peptide databases using a
single peptide sequence as query with BLASTP. |
To cite aLeaves
aLeaves
facilitates on-demand exploration of metazoan gene family trees on
MAFFT sequence alignment server with enhanced interactivity.
Shigehiro Kuraku,
Christian M. Zmasek, Osamu Nishimura, and Kazutaka Katoh.
Nucleic Acids
Research,
2013. 41 (W1): W22-W28.